Focus Workshop Slides 2011 June
Program of EBF Focus Workshop “Large meets Small” June 21-22, 2011
PDF renditions of the powerpoint presentations can be viewed and downloaded from this page by clicking the specific point on the program. The program itself can be viewed and downloaded by clicking here
Opening
New Technology Developments for Analysis of Large Molecules
- Lieve Dillen (Janssen R&D) – Comparison of different MS platforms for peptide quantification
- Debadeep Bhattacharya (Waters Corporation) – Implementation of a common high pH LC/MS/MS platform for small molecules and large molecules
- Kai Scheffler (Thermo Fisher Scientific) – Introducing a novel bench top Orbitrap for highly selective peptide/protein quantitation
- Lester Taylor (Agilent Technologies) – High Sensitivity Protein Quantitation Using a Triple Quadrupole with a Dual Ion Funnel
Cutting Edge Analytical Approaches – Peptides
- Lee Goodwin (Covance) – Strategies for the analysis of therapeutic peptides in biofluids
- Rachel Green (Quotient Bioresearch) – Comparison of clinical analyser and LC-MS/MS based approaches for quantification of serum IGF-I levels – Not Realeased for Publication
- Veniamin Lapko (Celerion) – Quantitative LC-MS/MS analysis of polypeptides: glucagon
- Hermann Mascher (pharm-analyt Lab. GmbH) – Quantification of selected peptides in biological fluids e.g. plasma
- Gerard Hopfgartner (University of Geneva) – The importance of sample preparation for the analysis of proteins and peptides by liquid chromatography with mass spectrometric detection
- Magnus Knutsson (Ferring Pharmaceuticals A/S) – LC-MS/MS bioanalysis of therapeutic peptides in plasma at low pg/mL levels
- Erin Chambers (Waters Corporation) – A selective SPE/LC-MS/MS assay for simultaneous quantitation of multiple amyloid beta peptides in cerebrospinal fluid
Validation Requirements for Large Molecule Analysis with MS Techniques
- Philip Timmerman (Janssen R&D for EBF) – First EBF reflections on Method validation criteria for peptide/protein analysis with LC-MS based techniques
- Gary Schulz (Advion Bioservices) – Validation of an immunoprecipitation, digestion and immunoaffinity LC-MS/MS assay for human NGF biomarker and implementation in support of clinical trials
- Nico van de Merbel (PRA International) – The use of internal standards for macromolecule quantification by LC-MS: lessons learned from small molecule assays
- Ben van Baar (QPS) – Validation requirements for a bioanalytical LC-MS/MS assay of trastuzumab
Cutting Edge Analytical Approaches – Proteins
- Yong-Xi Li (Medpace Bioanalytical Laboratories) – The powers of using MRM3 and high resolution MS with fast scan rates to conquer previously difficult large molecule compounds
- Olivier Heudi (Novartis Pharma AG) – LC-MS/MS quantification of proteins and peptides in biological fluids: towards a generic approach
- Stephanie Fischmann (Abbott) – High throughput method for the quantitative analysis of therapeutic antibody in the low ng/mL range from serum samples via affinity enrichment coupled LC-MS/MS to support clinical and preclinical PK/TK evaluation
- Ejvind Mørtz (Alphalyse) – Sample preparation for protein quantification by LC-MS/MS
- Ian Ward (Quotient Bioresearch) – Challenges in validating protein and peptide LC-MS/MS methodologies
- Alberto Guenzi (Hoffmann-La Roche) – Comparison of quantitative assays for a large protein: ELISA vs. LC-MS/MS
- Rand Jenkins (PPD) – Development and validation of an immunoaffinity-LC-MS/MS assay for 22 KDa somatropin (and hGH) in human serum
Analytical Strategies on Combining LBA and LC-MS(/MS) for Large Molecules
- Martin Nemansky (PRA International) – The sense and nonsense of quantitative analysis of biologics – or why should we care
- Brian Geist (Centocor) – Ligand Binding Assay critical reagent characterization via LC-MS
- Michael Adler (Chimera Biotec GmbH) – Highly sensitive Cytokine Quantification using Imperacer®, an Immuno-PCR based Ligand-Binding Assay System
Proteins in the MIST
- Michael Przybylski (University of Konstanz) – Ion mobility mass spectrometry: New molecular tool for the analysis of reactive intermediates in the oligomerization- aggregation of “misfolding”-aggregating proteins
- Richard Kay (Quotient Bioresearch) – Strategies for quantifying plasma protease susceptible peptides by LC-MS/MS – Not Released for Publication
- William van Dongen (PROXY laboratories) – Bioanalytical LC-MS of therapeutic oligonucleotides
- Filip Cuyckens (Janssen R&D) – Extracting metabolite ions of peptide drugs out of an in vivo matrix background
Closing
